What minimum volume of sample should be in a HPLC vial (2ml Im doing HPLC to identify and quantify polyphenols in my sample. My question is I prepare 2 ml of my sample in the glass vial ready for HPLC analysis and the injection volume is 20 ul (microlitre Sample Vials : Waters Waters also offers a selection of glass sample vials and plastic sample vials that fit most manufacturers’ instruments. Two of the most popular uses are as HPLC vials and GC vials. These chromatography vials can be u...

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How to clean used HPLC vials? - ResearchGate For vials, there are other really aggressive methods, Eg using diluted HF which will "eat" top layer of the glass. When I was undergrad student in analytical department we washed glass with HNO3 GOOD LABORATORY PRACTICE for HPLC recommended for HPLC use. Ultra pure HPLC water (18M Ω resistivity) is generated by passing deionized water through an ion exchange bed. Modern water purification instruments use this mechanism to produce water of suitabl...

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