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Choosing the Right Septum for High-Throughput Sample Preparation Workflows
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Apr 12, 2022 · Cryo-EM sample prep workflow. (Image credit: Thomas Warwick.) Figure 1 gives you an overview of the whole sample preparation process, how they are roughly categorized, and the optimizable parameters within each category. We’ll take the five categories as displayed and discuss them in more detail, albeit in a slightly different order.
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A PTFE/Silicone septum is provided with a thin 0.005” PTFE layer laminated to our high purity silicone and slit through the center to provide for easier needle penetration as well as to release the vacuum that forms when a large volume of sample is withdrawn from a vial. This septum provides chromatographic
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Nov 1, 2019 · However, due to almost nonreversible binding of proteins to C18 matrix, contamination and sample loss during the sample loading step might be a concern. Importantly, an iST-based 96-well device was also developed as a high-throughput sample processing platform and applied in clinical proteomics field [23]. Due to there are multiple steps for
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Aug 4, 2022 · The main benefits of automating your NGS sample prep workflow—namely, increased quality, throughput, and reliability—mean that you can have more confidence in your sequencing results. The DeNovix DS-11 Spectrophotometer – 1 µl full UV-Vis Analysis Designed by the original innovators of 1 µL UV-Vis spectrophotometery, the DeNovix DS
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specific sample requirements. • Durable yet flexible Slit septa cap has been specially designed for instruments with sensitive sampling needs. Applications • Use with standard robotics on HPLC instruments with sensitive needles, allowing for higher throughput. Slit Septa Mini-UniPrep Syringeless Filter For High Throughput Automation.
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aqueous based sample mixtures. Polypropylene septa offer better solvent compatibility compared to polyethylene, but piercing force is slightly higher. Viton Septa Viton septa are used in situations where a resealable septum is required for a sample matrix that aggressively attacks all other materials. Viton offers chemical resistance
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Oct 29, 2021 · The N2 chip reduces the sample processing volumes by one order of magnitude and allows over 5× more nanowells in one microchip for high-throughput single-cell preparation.
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Nov 10, 2021 · In recent years, MALDI MS has emerged as a high-throughput method with possible automation of both sample preparation and data acquisition. 384-well plates are prepared in <2 min using a bench-top
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May 12, 2023 · UmetaFlow is a workflow built for automated, high-throughput untargeted mass spectrometry-based metabolomics data processing and analysis using OpenMS algorithms. It allows for fast, scalable, and reproducible analysis through a workflow manager, Snakemake, but also for prototyping or smaller-scale interactive data processing through the
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Figure 1: RNA-seq library preparation workflow. After isolating total RNA from the sample of interest, cDNA is synthesized from the RNA, and several steps of purification and amplification are performed to yield a sequencing-ready RNA-seq cDNA library. Efficient removal of ribosomal RNA is critical for effective RNA-seq experiments.
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Use the simple online Thermo Scientific SureSTART Selection Guideto find the right septa and vial for your analysis. Thermo Scientific SureSTART Selection Guide. Answer a few simple questions and our SureSTART Selection Guide will recommend the best septa, cap, and vial for your workflow.
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HPLC & GC Certified Screw Vial & Cap Kits for 2 mL Samples. Precleaned, EPA certified assembled kits; includes 40 mL screw vials and 24 mm. open top screw caps. 0.3 and 1.5 mL screw top vial-cap kits for analysis of small sample volumes. For use with environmental analyses under EPA regulations.
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Laboratory instrumentation, chemistries and personalized support that simplify the process of automating sample prep in your lab. Includes modular nucleic acid purification solutions that let you adapt your laboratory workflow as your needs change.
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Oct 23, 2021 · The high-throughput workflow utilized the Evosep One system with a 21-min gradient and 4-min wash step, requiring 25 min to complete each sample. The mid-throughput workflow was run on an Ultimate 3000 ultra high-pressure chromatography system with a 60-min gradient and 12-min wash, and required 72 min to complete (see online Supplemental
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Summary overview of application-specific sample preparation and data analysis workflow recommendations Customer Collaboration –PacBio HiFiViral for SARSCoV-2 Workflow: High-Throughput Multiplexing 1.2 kb Amplicons for Full-Viral Genome Sequencing of SARS-CoV-2 (102-075-000 / 102-082-500) Technical documentation containing sample library
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