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Methods . Prospective and observational study. Neurotrauma intensive care unit of trauma center level I. Sixty consecutive, hemodynamically stable patients with severe traumatic brain injury, pre-transfusion hemoglobin < 10 g/dL, non-bleeding and monitored through intracranial pressure and brain tissue partial oxygen pressure (PtiO2) catheters were included.

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The HPLC vial is a seemingly minor component of an HPLC system but it has the potential to create major problems. This is particularly true for quantitative analysis. The following list of dos and don’ts will help you to avoid some of the more common pitfalls.

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High-performance liquid chromatography (HPLC) is an important analytical method commonly used to separate and quantify components of liquid samples. In this technique, a solution (first phase) is pumped through a column that contains a packing of small porous particles with a second phase bound to the surface.

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